Fig 2: EVI5L caused the G0/G1 phase arrest and promoted the S phase in the GMECs. (A) Pc-EVI5L and pcDNA3.1 were transfected in GMECs and the cells were cultured for about 24 h before collection, then the cell cycle was examined by FCM. (B) FCM analyzed the cell cycle of the GMECs transfected with miR-574-5p, si-EVI5L, and NC. (C,D) EVI5L affected the protein expression of the Rab23/ITGB1/TIAM1/Rac1 pathway in the WB assay. (E,F) Western blot analysis used to assess the effect of EVI5L on the TGF-ß/Smad pathway in GMECs. FCM: flow cytometry analysis; pc-EVI5L, overexpression EVI5L plasmid; pcDNA3.1: control group; NC: negative control; si-EVI5L: small interfering RNA of EVI5L; EVI5L +miR-574-5p: pcDNA3.1-EVI5L+miR-574-5p. ** means significant difference (p < 0.01), * means significant difference (p < 0.05).